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两株产纤维素酶细菌的分离和筛选
Isolation and selection of two strains of bacteria producing cellulase
【摘要】 从林区土壤中分离能够分解纤维素的细菌菌株共15株,采用刚果红染色的初筛平板获取透明圈较明显的菌株8株。在此基础上,进行液体培养并测定酶活,得到2株酶活较高的菌株,分别编号为X18和X10-1-2。这两株菌均为G(+)、形态杆状、可产芽孢、好氧,初步鉴定为芽孢杆菌属的两个不同种。
【Abstract】 The 15 strains of bacteria producing cellulase were isolated from soil.Eight colonies surrounded by some distinct zones of clearing in cellulose-congo red agar medium from the 15 strains above were obtained.By measuring the enzyme activity of liquid culture filtrates,two strains(termed X18 and X10-1-2,respectively) with higher cellulase activity were obtained.They are gram positive,endospore forming and aerobic bacteria.They all belong to the Bacillus genus.
【基金】 国家自然科学基金资助项目(50178021)
- 【文献出处】 黑龙江大学自然科学学报 ,Journal of Natural Science of Heilongjiang University , 编辑部邮箱 ,2007年02期
- 【分类号】Q93-3
- 【被引频次】10
- 【下载频次】515