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大孔吸附树脂富集三七总皂苷的工艺研究
Study on Purification of PNS with Macroporous Resin
【摘要】 目的:建立三七总皂苷的大孔吸附树脂吸附纯化的工艺条件及参数。方法:以三七总皂苷的含量为考察指标,对树脂吸附容量、洗脱溶酶及其用量进行了考察。结果:通过大孔吸附树脂富集与纯化后三七总皂苷洗脱率达80%以上,70%乙醇洗脱液干燥后的总固形物中三七总皂苷纯度可达81.6%。结果:三七提取物上D101大孔吸附树脂,以水洗脱后,以70%乙醇洗脱可以达到理想的结果。
【Abstract】 Objective:The technical parameters of PNS’s purification with resin were studied.Methods:PNS were purified by D101 macroporous resin and evaluated by yield.Results:Over 80% of PNS was eluted from macroporous resin.The purity was gathered in part of 70% ethanolic.Conclusion:This method is a easy,inexpensive,feasible one.
【关键词】 三七;
三七总皂苷;
比色法;
大孔吸附树脂;
【Key words】 Panax notoginseng; total saponins of Panax notoginseng(PNS); spectrometry; Macroporous resin;
【Key words】 Panax notoginseng; total saponins of Panax notoginseng(PNS); spectrometry; Macroporous resin;
- 【文献出处】 黑龙江医药 ,Heilongjiang Medicine Journal , 编辑部邮箱 ,2007年03期
- 【分类号】TQ461
- 【被引频次】9
- 【下载频次】450