【摘要】 目的研究不溶性镍盐(Ni3S2和Ni2O3)和水溶性镍盐(NiSO4)对细胞DNA的损伤状况,为镍致癌机制的研究提供科学依据。方法使用不同浓度Ni3S2、Ni2O3和NiSO4处理人胚肺成纤维细胞(humanlungfibroblast,HLF),通过单细胞凝胶电泳技术(SCGE)检测细胞DNA损伤程度。结果镍处理各组的HLF细胞较阴性对照组细胞DNA损伤程度增高(P<0.01),彗星尾矩增大(P<0.01),有一定的剂量-反应趋势。结论3种镍化合物均可引起细胞DNA损伤,不溶性镍盐的损伤强度大于水溶性的镍盐。DNA损伤是镍化合物致癌的可能机制。更多还原

【Abstract】 Objective To observe the DNA damage in the human lung fibroblasts(HLF)induced by insoluble nickel compounds(Ni3S2 and Ni2O3)and soluble nickel compound(NiSO4),and to study the mechanism of carcinogenesis.Methods The DNA damage of HLF cells treated with different concentrations of Ni3S2,Ni2O3 and NiSO4 were detected with the method of single cell gel electrophoresis(SCGE).Results All of the three kinds of nickel compounds significantly caused DNA damage in HLF cells,the average tail moments of all nickel-treated groups were larger than those in the control group(P<0.01).Conclusion Nickel compounds can induce DNA damage and the damage caused by insoluble compounds was much severely compared with soluble compounds.Maybe DNA damage is the possible mechanism of carcinogenesis induced by nickel compounds.更多还原