【摘要】 目的探讨氢醌(HQ)对体外培养L-02肝细胞DNA损伤及细胞周期的影响。方法将L-02肝细胞用不同浓度(0、5、10、20、40、80、160和320μmol/L)的HQ作用24h,采用噻唑蓝(MTT)比色法测定L-02肝细胞的相对存活率,采用单细胞凝胶电泳(SCGE)检测细胞DNA的损伤状况,并采用流式细胞术检测细胞周期分布状况。结果在0～80μmol/L的范围内,HQ对L-02肝细胞的存活率没有明显的影响(P>0.05);当染毒剂量超过160μmol/L时,其存活率则明显地下降(P<0.01)。随着HQ作用浓度的升高,L-02肝细胞DNA链的断裂程度也随着逐渐增加。0～80μmol/L范围内的HQ作用24h后,L-02肝细胞的细胞周期也发生了明显的改变,表现为S期细胞比例明显增加,G1期细胞的比例下降。结论HQ对L-02肝细胞DNA具有损伤作用,并且在体外能够引起明显的S期细胞阻滞。更多还原

【Abstract】 Objective To investigate the effects of hydroquinone(HQ)on DNA and cell cycle of L-02 hepatic cells in vitro.Methods After L-02 hepatic cells were exposed to HQ at the different concentrations(0,5,10,20,40,80,160 and 320 μmol/L)for 24 h,the cell survival rate was detected by MTT assay;DNA damage was detected by single cell gel electrophoresis(SCGE);the cell cycle distribution of L-02 hepatic cells treated with HQ were detected by flow cytometry.Results MTT assay showed that HQ of 0-80 μmol/L had little effect on survival rate of L-02(P>0.05),whereas the survival rate in each group of 160 and 320 μmol/L was significantly higher than that in the control(P<0.01)after 24 h treated with HQ.As the dose of HQ increased,the level of DNA damage increased.Treated with HQ(0-80 μmol/L)for 24 h,the distribution of cell cycle was altered,cells in S stage increased,while the cells in G1 stage decreased.Conclusion HQ may induce DNA damage and the cells arrested in S stage in the cultured L-02 hepatic cells.更多还原