【摘要】 隐孢子虫和贾第鞭毛虫是两种严重危害水质安全的病原性原生动物。美国国家环保总局(USEPA)制定并发布的1623方法是目前国际上最为常用的检测标准方法,其中,免疫磁性分离(IMS)是保证检测过程特异性的关键步骤。该文总结了IMS过程中回收率的主要影响因素,包括待测样品中的颗粒物质和溶解性物质,隐孢子虫和贾第鞭毛虫的浓度、育龄和活性等特征,以及磁珠添加比例、反应时间、反应体积、解离方式等操作参数。分析认为,将1623方法应用于环境样品或污水再生处理系统时,复杂的水质成分会显著影响IMS过程的回收效果,但可通过适当改进操作参数对IMS过程进行优化。更多还原

【Abstract】 Cryptosporidium and Giardia are two common species of pathogenic protozoan, seriously endangering the water quality. The most frequently used method for detection of Cryptosporidium and Giardia is named as "method 1623", which was edited and promulgated by USEPA. The specialty of method 1623 is mostly decided by immunomagnetic separation IMS, a key step of detection process. According to literature, particles and dissolved substances in water samples, concentration, age and viability of oocysts, amount of immunomagnetic beads, reaction time, reaction volume, and efficiency of dissociation between beads and oocysts all impact the recovery of IMS. Consequently, it is believed that complicated components of water samples from environment and waste water reuse system probably decrease the recovery of IMS. However, to improve the operation parameters could be a potential solvent of this problem.更多还原