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水和乙醇对DNA分子二维操纵的机制研究

The mechanism of 2-dimensional manipulation of DNA molecules by water and ethanol flows

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【作者】 申梓刚黄一波李宾张益

【Author】 SHEN Zigang HUANG Yibo LI Bin ZHANG Yi (Single Molecule Detecting and Manipulating Lab, shanghai Institute of Applied Physics,the Chinese Academy of Sciences, Shanghai 201800,China)

【机构】 单分子探测和操纵实验室中科院上海应用物理研究所单分子探测和操纵实验室中科院上海应用物理研究所 上海201800上海201800

【摘要】 DNA分子具有稳定的物理化学性质,成为纳米领域的重要实验材料,已有许多将其作为模板构建纳米线等方面的研究,因而,DNA分子的操纵技术显得尤为重要。分子梳技术利用水流动时的弯月面操纵DNA分子,是构建特定的DNA分子纳米结构的一个重要方法。本文采用一种改良分子梳技术,用原子力显微镜观察水和乙醇操纵DNA分子后形成的规则图案,分析弯月面和液流对DNA分子的作用,并讨论了液体和DNA分子在操纵时的作用机制。

【Abstract】 Due to its unique physical and chemical properties, DNA has recently become a promising material for building blocks in nanofabrication. Many researches focus on how to use DNA molecules as a template for nanowires. Molecular Combing technique is one of important methods to manipulate DNA molecules by using a water meniscus and form specific DNA nano-structures on surfaces. In this paper, by employing a modified molecular combing tech- nique, special patterns of DNA molecules was formed, and the interaction between liquid flows or meniscus and DNA molecules was analyzed, and the mechanism of manipulating DNA molecules by liquid was studied.

【关键词】 原子力显微镜(AFM)DNA操纵乙醇水流
【Key words】 Atomic force microscopy (AFM)DNAManipulationEthanolWater flow
【基金】 国家自然科学基金(10335070,10404032,30470441)资助
  • 【分类号】Q523
  • 【被引频次】1
  • 【下载频次】82
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