【摘要】 应用HPLC-MSn方法,研究了邻苯二甲酸丁基苄酯在小鼠尿液中的代谢产物,并对尿中推测的代谢物和标准品分别进行HPLC-UV和二级全扫描质谱(MS2)分析,结果在尿中发现了6个代谢产物,分别为邻苯二甲酸、马脲酸、邻苯二甲酸单丁酯、邻苯二甲酸苄酯、邻苯二甲酸单丁酯葡萄糖醛酸结合物及邻苯二甲酸苄酯葡萄糖醛酸结合物.推断环境激素邻苯二甲酸丁基苄酯在小鼠体内的代谢途径为原形首先水解生成邻苯二甲酸单丁酯、邻苯二甲酸苄酯、邻苯二甲酸,部分邻苯二甲酸发生脱羧反应生成苯甲酸,苯甲酸再与内源性物质甘氨酸结合成马脲酸,邻苯二甲酸单丁酯、邻苯二甲酸苄酯和内源性β-D-葡萄糖醛酸结合,生成水溶性较大的邻苯二甲酸单丁酯葡萄糖醛酸结合物和邻苯二甲酸苄酯葡萄糖醛酸结合物.更多还原

【Abstract】 The metabolites of butylbenzyl phthalate in mice urine were identified by HPLC-MS~n . After the analysis of speculated metabolites and standard sample by HPLC-UV and the second full mass scanning (MS~2), we find six metabolites: phthalic acid, benzoyl glycocoll, monobutyl phthalate, benzyl phthalate, glucuronyl-monobutyl phthalate, glucuronyl-benzyl phthalate. We deduced metabolic approach of butyl benzyl phthalate in vivo was that butyl benzyl phthalate hydrolyzed into the phthalic acid, monobutyl phthalate and benzyl phthalate. Partial phthalic acid became into benzoic acid by decarboxylizing. Then benzoic acid conjugated endogenous glycin and benzoyl glycocoll was produced. Monobutyl phthalate and benzyl phthalate conjugated endogenous β-D-glucuronic acid and produced the glucurony-monobutyl phthalate and glucuronyl-benzyl phthalate.更多还原