【摘要】 通过富集培养,从红树林底泥中分离出6株硝基苯酚降解菌,其中Achromobacter xylosoxidansNS12在好氧条件下可耐受小于1.8 mmol/L的邻硝基苯酚(ONP)或3.0 mmol/L的对硝基苯酚(PNP),能以PNP和ONP作为唯一碳源、能源和氮源生长并将其完全矿化,但该菌不能利用间硝基苯酚(MNP)作为唯一碳源和氮源生长.研究发现A.xylosoxidansNS12在降解PNP和ONP组成的混合底物时,PNP的存在可抑制ONP的降解,同时ONP的存在也抑制PNP的降解.此外,在利用PNP和ONP的混合底物时,NS12转化PNP的速率显著地高于转化ONP的速率.红树林底泥中固有的细菌对PNP和ONP具有高效降解作用.更多还原

【Abstract】 A nitrophenols-degrading bacterium,strain NS12,was isolated from a mangrove sediment by enrichment culture under aerobic conditions.Based on the analysis of 16S rDNA gene sequence the isolate was identified as Achromobacter xylosoxidans Strain NS12 was able to metabolize both o-nitrophenol(ONP) and p-nitrophenol(PNP) as the sole source of carbon,energy and nitrogen.However,this strain was not able to use 3-nitrophenol(MNP) as the only source of carbon energy and nitrogen for growth.The study demonstrated that when PNP and ONP occurred as a mixed substrate PNP degradation restrained the degradation of ONP and caused the major carbon source shift from ONP to PNP.Moreover,the results showed nitrophenols could be degraded by the indigenous bacteria in mangrove sediment.更多还原