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利用亚硝酸盐为电子受体反硝化聚磷菌的筛选与富集

Screening and enrichment of denitrifying phosphorus accumulating bacteria using nitrite as electronic acceptor

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【作者】 周康群刘晖孙彦富周遗品刘洁萍

【Author】 Zhou Kangqun Liu Hui Sun Yanfu Zhou Yipin Liu Jieping(Department of Environmental Science and Engineering,Zhongkai Agrotechnical College,Guangzhou 510225)

【机构】 仲恺农业技术学院环境科学与工程系仲恺农业技术学院环境科学与工程系 广州510225广州510225

【摘要】 依据DPB原理,利用SBR动态反应器和静态释/聚磷装置。以A2/O工艺厌氧段污泥为种泥,研究以亚硝酸盐为电子受体反硝化聚磷菌的筛选与富集,同时对选择、富集污泥的反硝化聚磷性能进行了考察。结果表明:利用亚硝酸盐为电子受体的反硝化聚磷菌存在于A2/O厌氧段污泥中,通过厌氧/好氧和厌氧/缺氧方式运行后,聚磷菌总数由1400个/mL增加到32 000个/mL,其中反硝化聚磷菌占聚磷菌总数的比例也由14.5%提高到81%,磷酸盐和亚硝酸盐去除率分别由最初的8.65%和7.55%上升到91%和95.62%;筛选与富集利用亚硝酸盐为电子受体的反硝化聚磷菌时,缺氧段进水COD的浓度须控制在10 mg/L以下;当体系处于稳定状态,且亚硝酸盐氮浓度高达30 mg/L时,并未对反硝化聚磷菌的生存产生抑制和体系运行产生干扰,此时磷酸盐出水低至1.06 mg/L。

【Abstract】 In this paper,according as the denitrifying phosphorus bacteria(DPB) principle,dynamic SBR reactor and static releasing/accumulating phosphorus installation were investigated.The anoxic sludge of A2/O technology was used as inoculum.The screening and enrichment of denitrifying phosphorus accumulating bacteria(DPB) using nitrite as electronic acceptor and its characteristics were studied.The result shows: the DPB using nitrite as electronic acceptor indeed lies in the anoxic sludge of A2/O technology.After anaerobic/aerobic and anaerobic/anoxic stages,the amount of PAO increases from 1400 to 32 000.The ratio of DPB to PAO improves from 14.5% to 81%.The removal rates of phosphate and nitrite rise from 8.65% and 7.55% to 91% and 95.62% respectively;when the DPB using nitrite as electronic acceptor is screened and enriched,the COD concentration of influent in the anoxic stage must be controlled below 10 mg/L.The existence of DPB is not restrained and the system is not disturbed when the system is stable and the nitrite concentration reaches as high as 30 mg/L.At the same time the concentration of phosphate in the effluent is below 1.06 mg/L.

【基金】 广东省自然科学基金资助项目(06022869)
  • 【文献出处】 环境工程学报 ,Chinese Journal of Environmental Engineering , 编辑部邮箱 ,2007年08期
  • 【分类号】X703
  • 【被引频次】17
  • 【下载频次】660
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