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有机相中刻录脂肪酶聚合物对配体的吸附

Adsorption of ligand on recorded lipase polymers in organic phase

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【作者】 宋锡瑾宣锋王杰龚伟

【Author】 SONG Xijin1, XUAN Feng1,WANG Jie2 , GONG Wei1(1Department of Chemical & Biochemical Engineering, Zhejiang University, Hangzhou 310027,Zhejiang, China; 2Department of Chemistry, Zhejiang University, Hangzhou 310027, Zhejiang,China)

【机构】 浙江大学化学工程与生物工程学系浙江大学化学系浙江大学化学工程与生物工程学系 浙江杭州310027浙江杭州310027

【Abstract】 Lipase and lauric acid were added into a copolymer system, which contained PEG400-dimethacrylate, trimethylolpropane trimethacrylate, and a surface-active agent.The polymerization was initiated by ultraviolet irradiation .Then recorded lipase polymers (RLP) of locking lipase conformation were obtained after lauric acid was extracted by a neat organic solvent.The adsorption of RLP in the lauric acid solution was studied.The adsorption isothermal curves were measured at temperature 30—50℃.The results indicated that RLP’s adsorption capacity was far stronger than blank carrier’s and immobilized lipase polymers (ILP)’s,about 8.7 and 3.9 times respectively.The adsorption process followed the Langmuir and Freundlich isothermal adsorption equations.

【关键词】 脂肪酶月桂酸构象刻录吸附
【Key words】 lipaselauric acidconformationrecordadsorption
【基金】 国家自然科学基金项目(20276067)~~
  • 【文献出处】 化工学报 ,Journal of Chemical Industry and Engineering(China) , 编辑部邮箱 ,2007年05期
  • 【分类号】O647.3
  • 【被引频次】3
  • 【下载频次】69
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