【摘要】 目的本研究探讨内皮固有型一氧化氮合酶(ecNOS)基因第4内含子27bp可变数目串联重复序列(VNTR)插入/缺失多态性(4b/a)与2型糖尿病(T2DM)并发冠心病的关联性。方法观察80例2型糖尿病、92例T2DM合并冠心病患者及119例正常对照组人群,提取基因组DNA并应用PCR技术检测ecNOS基因内含子4VNTR多态性。结果ecNOS基因4b/a多态性与T2DM及T2DM并发冠心病无关。发现1例4a/a纯合子基因型者,位于T2DM并发冠心病组。结论缺失型等位基因4a可能处于非统治地位,是否与T2DM并发冠心病有关仍待更大样本的观察。更多还原

【Abstract】 Objective To investigate the relationship between polymorphism of intron 4 (4b/a) of the end othelial constitutive nitric oxide synthase(ecNOS) gene and type 2 diabetes mellitus(DM) complicated with coronary hear tdisease.Methods 80 patients with type 2 DM(Group DM),90 patients with type 2 DM complicated with coronary heart disease(Group DH))and 119 healthy subjects (Control group) were observed in this study.Genome DNA was extracted from anticoagulant blood. The polymorphism 4b/a was determined by polymerase chain reaction(PCR).Results The polymorphism of intron 4 (4b/a) of ecNOS genome was not correlated with type 2 DM or type 2 DM complicated with coronary heart disease. There was only one homozygote 4a/a patient in group DH.Conclusion The allele 4a might be in non-dominant position,and whether which is related with type 2 DM complicated with coronary heart disease need to beobserved in more specimens further.更多还原