【摘要】 目的:探讨总抗氧化能力、丙二醛、脂质过氧化物对诊断超声照射后大鼠生殖细胞凋亡的影响。方法:SD雄性健康大鼠32只,用诊断超声照射不同时间,24 h后取大鼠睾丸组织,用生物素-dUTP酶标亲和素法(TUNEL法)检测细胞凋亡率(AI),化学比色法测定总抗氧化能力(T-AOC)、丙二醛(MDA)、脂质过氧化物(LPO)。结果:诊断超声照射10 min组,睾丸组织中T-AOC(IU/g)为36.4±4.6、MDA(μmol/g)为1.62±0.53、LPO(μmol/g)为1.83±0.35、睾丸生殖细胞凋亡(%)为6.6±0.35,分别与对照组(34.5±3.5,1.25±0.45,1.78±0.27,5.8±0.21)比较,差异无统计学意义(均为P>0.05),而超声照射20 min组T-AOC(IU/g)为18.3±2.8、MDA(μmol/g)为8.56±0.68、LPO(μmol/g)为7.36±0.28、细胞凋亡(%)为16.3±3.67;30 min组的睾丸组织中T-AOC(IU/g)为16.2±1.7、MDA(μmol/g)为8.13±0.75、LPO(μmol/g)为7.28±0.37、细胞凋亡(%)为18.5±2.89。超声照射20 min组及30 min组T-AOC显著降低,MDA、LPO含量升高,睾丸生殖细胞凋亡增加,与对照组比较,差异有统计学意义(均为P<0.01)。结论:诊断超声照射20 min以上,可导致大鼠睾丸生殖细胞凋亡增加,且与睾丸组织中总抗氧化能力下降、丙二醛及脂质过氧化物升高密切相关。更多还原

【Abstract】 Objective: To study the effect of oxidation/antioxygen system on the germ cell apoptosis after diagnostic ultrasound radiation.Methods: Thirty-two immature male healthy rats(Sprague-Dawley) were administered diagnostic ultrasound radiation for different time.The levels of total antioxidant capacity(T-AOC),malondialolehyde(MDA) and lipidperoxide(LPO) in testis were(evaluated) by spectrophotometric method.Germ cell apoptosis rates were detected by TdT-mediated dUTP-x nick end labeling(TUNEL).Results: The level of T-AOC(IU/g),MDA(mol/g),LPO(μmol/g) and the apoptosis rate(%) was 36.4±4.6,1.62±0.53,1.83±0.35 and 6.6±0.35 respectively in 10-min-irradiation group;the indices were 18.3±2.8,8.56±0.68,7.36±0.28 and 16.3±3.67 respectively in 20-min-irradiation group;16.2±1.7,8.13±0.75,7.28±0.37 and 18.5±2.89 respectively in 30-min-group;and 34.5±3.5,1.25±0.45, 1.78±0.27,and 5.8±0.21,in the control group.There were significant differences between each of the groups for irradiating for at least 20 minutes and the control group in these indices(all P<0.01).While no significant difference between the 10-min-irradiation group and control group was found(all P>0.05).Conclusion: If the time of diagnostic ultrasound radiation is beyond 20 minutes,testicle apoptosis rate is increasing along with the descending antioxygen capability,MDA and LPO.更多还原