【摘要】 观察去铁胺(DFO)对原代培养的心肌细胞铁代谢的影响,探讨DFO对心肌细胞铁代谢的影响机制.用原代培养的乳鼠心肌细胞为材料,以不同浓度的DFO孵育细胞,然后检测心肌细胞存活率、搏动频率以及铁转运相关蛋白CP,HP,FP1的表达变化.结果表明:各剂量DFO对心肌细胞存活率无明显影响;心肌细胞搏动频率减慢,停止跳动的细胞数量明显增加,收缩幅度逐渐降低;随着DFO浓度的增加,心肌细胞CP和HP的表达增加而FP1表达减少.由此可以看出,DFO不影响心肌细胞的存活率,但影响细胞的生活状态,也影响心肌细胞内CP,HP和FP1蛋白的表达.更多还原

【Abstract】 To observe the effect of DFO on iron metabolism in primary cultured rats’ cardiac myocytes.Newborn rat cardiac myocytes were isolated and cultured with the medium containing DFO.It was determined the cells’ livability,beat rhythmically and beat amplitude of cardiac myocytes were examined.The expression of CP,HP and FP1 was also examined.Results showed that compared with control,DFO had no clearly influence on cells’ livability,but it could decrease cells’ beat rhythmically and beat amplitude.CP and HP expression increased after the incubation of DFO,however,FP1 expression decreased.DFO has no influence on cardiac myocytes’ livability,but it could affect the cells’ living state.DFO could regulate and control expression of iron transport proteins.更多还原