【摘要】 昆虫细胞-杆状病毒表达体系给生物杀虫剂的工业化生产带来了希望。为了提高杆状病毒在昆虫细胞中的复制量并大规模生产生物杀虫剂,高性能、低成本培养基的选取十分必要。将Grace’s培养基与IPL-41及Schneider’s培养基的组分进行比较后,以Grace’s为基础,向其中加入酵母提取物、水解乳蛋白、氨基酸以及钼、锰等微量元素进行改善,用于培养细胞HzAm1,最大细胞浓度提高了190.2%,改进效果明显。更多还原

【Abstract】 Insect cell-baculovirus expression system is emerging a hopeful future for the industrial production of bio-insecticides.In order to improve the baculovirus replication in insect cells and be feasible in bio-insecticide production on large scale,the selection of high-performance and low-cost medium is quite necessary.In this study,compared to media IPL-41 and Schneider’s,medium Grace’s is improved through the addition of yeast extract,lactalbumin hydrolysate,amino acids,manganese,molybdenum and etc.It is indicated that the insect cells HzAm1 concentration is greatly raised by 190.2% when cultivated with this modified Grace’s medium.更多还原