【摘要】 建立一种准确的测定全血铁、锌、镁的新方法.全血样经过硝酸—硫酸—高氯酸消化后,采用微量注射进样-火焰原子吸收法测定了Ⅱ型糖尿病患者全血中铁、锌、镁的含量.实验结果为3种元素的线性范围分别是5.0~25,0.1~1.0,0.5~4.0 mg/L,相关系数r均在0.999以上,平均回收率分别为99.86%,94.88%,99.60%,相对标准偏差分别为1.40%,4.83%,1.63%,检出限分别为0.210 7,0.029 8,0.014 6 mg/L;用此方法测定104名Ⅱ型糖尿病患者的铁、锌、镁含量平均值分别为197.9±60.26,4.808±1.949,23.35±7.731 mg/L,且均具有显著性差异(P<0.001).实验证明该法具有样品需要量小、灵敏、准确、精密、快速、廉价等特点,适用于现代医学研究和临床检测.更多还原

【Abstract】 A new and accurate assay method for the determination of iron,zinc and magnesium concentration in whole blood was establshed. First,whole blood samples were digested by nitric acid-sulfuric acid-perchloric acid.Then,iron,zinc and magnesium concentration was examined by FAAS with Micro Injection technique.The linearity of the three elements were 5.0～25,0.1～1.0,0.5～4.0 mg/L,respectively,r>0.999.The recoveries of whole method were 99.86%,94.88%and 99.60% respectively.Relative standard deviations were 1.40%,4.83% and 1.63% respectively.The detecting limits were 0.210 7,0.029 8,0.014 6 mg/L respectively.The average value of iron,zinc and magnesium in whole blood of the Ⅱ Diabetes Mellitus were 197.9±60.26,4.808±1.949,23.35±7.731 mg/L respectively.The content of three elements for the Ⅱ Diabetes Mellitus are lower than the healthy people and the difference is remarkable.The characteristic of this method were single sampling with small volume blood,the sensitivity,accuracy,precision,quickness and low-cost as well.This method is suitable to be used in clinical laboratories.更多还原