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中华眼镜蛇毒抗瘤多肽对人卵巢癌HO-8910细胞的增殖抑制和Fas/FasL表达的影响

Inhibition of proliferation and influence of Fas/FasL expression by antitumor polypeptide from naja actra venom in HO-8910 cell

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【作者】 戚潜辉陈盛强宋绿茵李锦玉

【Author】 Qi Qianhui,Chen Shegqiang,Song Luyin,Li jinyu.Department of Gynecology and Obstetrics,the Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510260,China

【机构】 广州医学院第二附属医院妇产科广州医学院第二附属医院神经研究所广州医学院第二附属医院妇产科 510260510260

【摘要】 目的探讨中华眼镜蛇毒抗瘤多肽对人卵巢癌HO-8910细胞株的抑制作用及Fas/FasL表达的影响。方法用MTT比色法检测不同浓度中华眼镜蛇毒抗瘤多肽对HO-8910细胞的增殖抑制,RT-PCR和免疫组织化学观察原癌基因Fas/FasL表达的变化。结果中华眼镜蛇毒抗瘤多肽对HO-8910细胞增殖抑制作用成剂量依赖性,RT-PCR和免疫组化结果显示,随着中华眼镜蛇毒抗瘤多肽作用剂量的增加,Fas/FasL基因的表达无明显变化。结论中华眼镜蛇毒抗瘤多肽对人卵巢癌HO-8910细胞系的增殖具有明显的抑制作用,并对原癌基因Fas/FasL的表达无抑制作用。

【Abstract】 Objective This study was designed to investigate the inhibition of antitumor Polypeptide from Naja Actra Venom(APNAV) on HO-8910 cell line and its mechanism.Methods MTT was used to measure the levels of the proliferation of HO-8910 cultured with APNAV in different concentrations.The effects of apotosis on cell to HO-8910 were observed by DNA Ladder.The expression of Fas/FasL was measured by RT-PCR and immunohistochemical technology.Results The proliferation of HO-8910 was obviously inhibited by APNAV in a dose dependent manner.The outcome showed that the expression of gene Fas/FasL was not notably changed,when the dose of APNAV was increased.Conclusion APNAV can inhibit the proliferation of HO-8910 and can not affect the expression of gene Fas/FasL.

【基金】 广州市中医药中西医结合科研项目(编号2004A062);广东省科技厅社会发展与基础研究项目(编号2004-44)
  • 【文献出处】 广州医药 ,Guangzhou Medical Journal , 编辑部邮箱 ,2007年01期
  • 【分类号】R737.31
  • 【被引频次】3
  • 【下载频次】95
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