【摘要】 目的研究鼻咽癌组织照射2 Gy后基因表达谱的变化。方法采用B ioStarH-141 s(2004)型含14 112点的人类全长基因cDNA表达谱芯片,对1例放射敏感的鼻咽癌组织进行放疗前及放疗2 Gy后检测,并分析它们之间的基因表达差异。结果鼻咽癌放疗2 Gy后与放疗前比较,组织表达差异基因共38个,其中上调28个,下调10个。表达差异基因中改变最明显的功能群是免疫系统相关的基因,如IGLV 1-44、SLPI、CXCL 14、IL1RN、HLA-DQA 1、HLA-DOA等上调。细胞增殖、细胞调亡、细胞周期、DNA修复系统也有部分基因发生明显变化,如MLL 5上调、POLR2B下调等。结论提示对鼻咽癌组织照射2 Gy后基因表达谱改变的研究可以更好地阐明放射敏感性差异机理,为放疗前或放疗早期寻找到预测放射敏感性分子标志提供理论依据。更多还原

【Abstract】 Objective To study the change of gene express profile of nasopharyngeal carcinoma(NPC) tissue before and after the radiotherapy of 2 Gy.Methods BioStarH-141s(2004) profile genechip which contained 14112 points of full length human genes was used to detect one NPC tissue before and after radiotherapy and to analyse the genes expressed differently.Results The results showed that,after radiotherapy of 2 Gy,there were 38 genes expressed differently,including 28 up-regulate genes and 10 down-regulate genes.And the gene group which changed most obviously was related to the function of immunity,for instance,up-regulate genes:IGLV1-44、SLPI、CXCL 14、IL1RN、HLA-DQA 1、HLA-DOA.Some genes related to cell proliferation,apoptosis,cell cycle and DNA-repair system,also differently expressed in evidence,for instance,up-regulate gene:MLL 5,and down-regulate gene:POLR2B.Conclusion The results indicated that,research about the change of the gene express profile of NPC tissue before-and-after the radiation could better clarify the mechanism of radiation sensitivity.It may afford theoretic idea to find molecular markers to predict radiation sensitivity before or at the forepart of radiation therapy.更多还原