【摘要】 目的了解广西仫佬、壮和汉三民族人群中HIV辅助受体CCR5△32和CCR5-894C缺失等位基因突变频率和多态性的特点,为评估这三个民族人群对HIV的遗传易感性和艾滋病的防治提供理论依据。方法以197例仫佬族,100例壮族和100例汉族为研究对象,应用PCR和DNA测序等方法检测CCR5△32和CCR5-894C缺失突变体。结果未发现CCR5△32和CCR5-894C缺失突变体,均为野生型。结论由于未发现CCR5△32和CCR5-894C缺失突变体,推测广西仫佬、壮和汉三民族人群对HIV-1病毒感染可能具有较大的遗传易感性。更多还原

【Abstract】 Objective To analyze the mutation frequency of CCR5△32 and CCR5-894C deletion allele and the characters of polymorphism,and evaluate of the sensitvity of infection of those multi-national residents to AIDS.Methods Our analysis was based on 197 blood-samples of Mulao people,100 blood-samples of Zhuang people and 100 blood-samples of Han people. The mutant of allele in CCR5△32 and CCR5-894C was analyzed by PCR method and sequence analysis. Results No mutant of allele in CCR5△32 and CCR5-894C was found in those 397 samples tested. All belonged to the type of wild. Conclusion After series of inspections in the frequency of mutant of HIV-l co-receptor CCR5△32 gene and CCR5-894C deletion allele in Mulao, Zhuang and Han, no mutant was found. We can make a conclusion that the people of Mulao, Zhuang, and Han in Guangxi Zhuang Autonomous Region face great genetic possibility in infection of HIV-1.更多还原