【摘要】 目的:研究慢性肝病患者乙型肝炎病毒核心基因启动子(HBVBCP)变异与临床类型的相关关系。方法:采用PCR微板核酸杂交结合ELISA检测显示技术,对176例HBV慢性感染患者的血清进行HBVBCP区核苷酸(nt)1762碱基A→T和1764G→A联合突变检测。根据病情的严重程度将患者分为6组不同临床类型:①慢性乙型肝炎轻度;②慢性乙型肝炎中度;③慢性乙型肝炎重度;④肝炎肝硬化;⑤慢性重型肝炎;⑥原发性肝癌。并与HBVBCP变异的阳性或阴性进行等级相关分析。结果:HBVBCP双变异在慢性乙型肝炎轻、中、重度组,肝炎肝硬化组,慢性重症肝炎组和原发性肝癌组的阳性率分别为28.0%、31.4%、34.2%、39.4%、60.0%和70.0%(rs=0.259,P=0.001)。结论:HBVBCP变异与HBV慢性感染的由轻至重的临床类型呈正相关,随着病情的加重,BCP变异的阳性率亦随之增高。更多还原

【Abstract】 Objective: To study the correlation between hepatitis B virus (HBV) basic core promoter (BCP ) mutation and clinical type in patients with chronic hepatitis B virus infection. Methods:176 patients with chronic hepatitis B virus infection were detected for A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1764 by the method of polymerase chain reaction (PCR) microplate hybridization ELISA. They were divided to six groups by pathogenetic condition (Chronic hepatitis B with mild, moderate and sever; liver cirrhosis, Chronic fulminant and HCC). The six groups were assigned to 1～6,then ranked in correlation to the positive or negative of (BCP) mutation. Result: The positive rates of HBV BCP mutants in chronic hepatitis B were mild , moderate and severe; liver cirrhosis, chronic fulminant hepatitis and HCC were 28.0%,31.4%,34.2%,39.4%,60.0%和70.0% respectively. There were positively correlations between HBV BCP mutants and the progression of HBV-related liver disease(rs=0.259, P=0.001).Conclusion: The positive rate of HBV BCP mutants was more highly associated with the development of progressive liver diseases.更多还原