【摘要】 目的:扩增广西眼镜蛇神经生长因子(NGF)的全长基因并进行序列分析。方法:应用聚合酶链反应技术,以广西眼镜蛇cDNA为模板,扩增出编码NGF的全长基因,并克隆至pMD18-T载体中进行基因测序。结果:所得序列与Genbank提供的序列(M61180)对比显示,广西眼镜蛇prepro-NGF与Naja kaouthia(monocled cobra)、Bungarus multicinctus、mouse、人的NGF同源性分别为99%、91%、66%、68%,将不同氨基酸相同的化学性质计算在内,同源性更高。结论:成功地获得了广西眼镜蛇NGF的全长基因,且序列与已知序列高度一致。更多还原

【Abstract】 Objective: To amplify and sequence nerve growth factor (NGF) gene from Naja naja atra. Methods: NGF was obtained by polymerase chain reaction by using Naja naja atra genomic DNA as a template and cloned into pMD18—T vector and sequenced. Result: The sequence of NGF was compared with those of the other snake venom(Naja kaouthia (monocled cobra),Bungarus multicinctus,mouse and human. The proteases of the sequence were aligned to maximize mutual homologies(99%,91%,66%,68% respectively).Conclusion: NGF gene from Naja naja atra was successfully obtained and its sequence was highly consistent with the ones reported.更多还原