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猪笼草组培快繁技术研究

Tissue culture and rapid propagation of Nepenthes mirabilis

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【作者】 谢怡青谢雄辉丘亮伟黄愉光

【Author】 XIE Yi-qing,XIE Xiong-hui,QIU Liang-wei,HUANG Yu-guang(Meizhou Institute of Agricultural Sciences,Meizhou,Guangdong 514011,China)

【机构】 梅州市农业科学研究所梅州市农业科学研究所 广东梅州514011广东梅州514011

【摘要】 以猪笼草的顶芽和腋芽为外植体进行组培快繁研究,结果表明:在1/2MS+6-BA 1mg/L+NAA 0.1mg/L培养基中培养10d后,能诱导出幼芽;1/2MS+6-BA 2mg/L+NAA 0.1mg/L培养基对丛生芽的增殖效果最好,增殖倍数达4.1;生根培养基以1/4MS+6-BA 0.1mg/L+NAA 0.5mg/L为最佳,生根率可达95%以上。

【Abstract】 The top buds and axillary buds of Nepenthes mirabilis used as explants for tissue culture and rapid propagation were studied.The results showed that the young buds could be induced after 10 days cultured on the medium 1/2MS+6-BA 1mg/L+NAA 0.1mg/L.The 1/2MS medium with 6-BA 2.0mg/L and NAA 0.1mg/L was the best for multiplication culture of clumpy buds and the multiplication coefficient was 4.10.The 1/4MS medium with 6-BA0.1mg/L and NAA 0.5mg/L was the best for inducing roots from shoots and the rootage ratio was above 95%.

【关键词】 猪笼草组织培养快速繁殖
【Key words】 Nepenthes mirabilistissue culturerapid propagation
  • 【文献出处】 广西农业科学 ,Guangxi Agricultural Sciences , 编辑部邮箱 ,2007年02期
  • 【分类号】S682.3
  • 【被引频次】2
  • 【下载频次】540
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