【摘要】 用纯化的可溶性重组resistin蛋白免疫健康家兔,制备抗血清,优化ELISA各反应条件(如工作浓度、温育时间、特异性试验),初步建立了检测猪resistin蛋白的间接ELISA方法。结果表明抗原、抗体最佳工作浓度为1∶200和1∶400,resistin最低检出限量为193.75 ng/ml;抗原和一抗、一抗和二抗的最适温育时间均为60 min。本试验建立的ELISA方法,具有灵敏度较高,特异性较好的特点,可作为猪组织和血清中resistin蛋白的检测方法。更多还原

【Abstract】 The purified resistin was used as antigen for immunizing rabbit to obtain resistin specific antisera.The ELISA for resistin detection is developed initially by optimizing conditions of ELISA action.The results showed that the working concentration of antigen-antibody was 1∶200 and 1∶400, the lowest detectability of resistin was about 193.75 ng/ml.The established ELISA method was shown to be sensitive,specific and reproducible.更多还原