【摘要】 为了测定猪卵巢组织磷酸二酯酶(PDE)同工酶基因表达类型与活性规律,研究相关中药的作用机理,为繁殖障碍性疾病治疗积累资料。作者提取中国实验用小型猪卵巢组织总RNA,应用反转录聚合酶链反应(RT-PCR)测定PDE同工酶在其中的表达。选取催情散等复方及单味药,运用高效液相色谱法(HPLC)根据作用前后cAMP/cGMP含量的变化,分析对PDE活性的影响。结果检测的18个PDE亚型中除PDE4C外,PDE 1A、1B、1C、2A、3A、3B、4A、4B、4D、5A、7A、7B、8A、8B、9A、10A、11A mRNA在卵巢组织中均有表达。卵巢组织PDE活性较强,其中cGMP-PDE活性大于cAMP-PDE活性。催情散对卵巢组织cGMP-PDE活性均有较强的抑制作用,其中单味药淫阳藿作用最强。研究结果表明卵巢组织含有丰富的PDE同工酶,在通过环核苷酸水平卵巢机能调节中发挥着重要作用,中药催情散的作用与显著抑制cGMP-PDE活性有关。更多还原

【Abstract】 Objective: To detect gene express and activity of phosphodiesterase(PDE) existed in porcine ovary and to study the mechanism of relative herbs for the data accumulation of breed disorder diseases.Method:PDE gene expression was detected by RT-PCR after the extraction of total RNA from the ovary of China experimental miniature pig;prescriptions like Cuiqing San and single herb were selected and the influence on PDE activity was analyzed,which was calculated by cAMP/cGMP content change examined with HPLC before and after the reaction.Results: mRNAs of PDE1A,1B,1C,2A,3A,3B,4A,4B,4D,5A,7A,7B,8A,8B,9A,10A,11A were expressed in porcine ovary except for mRNA of PDE4C in 18 subtypes examined;there was strong PDE activity in the ovary,and the activity of cGMP-PDE was stronger than that of cAMPPDE.Each herb in Cuiqing San had powerful inhibition on cGMP-PDE activity,and Yinyanghuo was the most powerful herb.Conclusion:There are abundant PDE isoenzyme in porcine ovary,which exert important effect in the regulation of ovary function by cyclic nucleotide level,and the action of Cuiqing San is bound with its significant inhibition on cGMP-PDE activity.更多还原