【摘要】 为探讨硝基苯中毒所致乙酰胆碱酯酶的变化规律,本试验用不同剂量的硝基苯对小鼠进行灌胃染毒,1次/d,共30 d,采用碱性羟胺比色法测定小鼠脑乙酰胆碱酯酶活性和肝乙酰胆碱含量,并在试验期间记录小鼠的临床表现。结果表明:染毒期间小鼠表现为行动迟缓、反应迟钝、抽搐及情绪异常如兴奋、敏感、不停跳跃等症状,随着染毒剂量的增加,时间的延长,中毒症状越明显;乙酰胆碱酯酶活性表现为明显的抑制作用,肝乙酰胆碱含量增加。结论:硝基苯具有神经毒性,能够抑制肝和脑乙酰胆碱酯酶活性,且表现为明显的剂量效应。更多还原

【Abstract】 In order to explore the changing rule of the cholinesterase caused by nitrobenzene poisoning.Once a day,we fed mice with different doses of cholinesterase 30 days in all.The activity of cholinesterase in brain and the quantity of cholinesterase in liver were detected by alky hydroxylamine chromometry.The clinical manifestation of mice were observed during the test.The results indicated that mice show various symptomes such as slow action,obtuse reaction,hyperspasmia and abnormal emotion for example excition,saitivity and continual jump.These symptomes are increasingly obvious with the progressive degree of intoxation and time passing.The activity of cholinesterase in brain was depressed while the quantity of cholinesterase in liver increasing.We concluded that nitrobenzol is neurovirulent and it is able to inhibit the activity of cholinesterase in both liver and brain which displays evident dosage effect.更多还原