【摘要】 RNAi主要通过双链RNA(dsRNA)被核酸酶切割成21～23nt的干涉性小的RNA,即siRNA,由siRNA介导识别并靶向切割同源性靶mRNA分子而实现的;RNAi需要多种蛋白质因子以及ATP参与,而且具有生物催化反应特征;RNAi具有高效性和高度特异性,作为一种关闭特定基因的新技术,为基因功能研究提供了一个快速、简便的方法,在后基因组时代应用前景广阔。更多还原

【Abstract】 RNA interference(RNAi) is a post-transcriptional gene silencing induced by introduction of double-stranded RNA (dsRNA)。RNAi is achieved through incising by nucleic acid into small inference RNA of 21~23 nt by dsRNA, which is called siRNA, and is recognized and targeting incises the homologous mRNA molecular. RNAi needs various protein factors as well as ATP to participate in. It posesses the character of biocatalyzing, high efficiency and speciality. It has provided a fast and easy way for the study of the gene function as a new technology of shutting down fixed gene, which has an optimistic foreground in the post-genome time.更多还原