【摘要】 应用气相质谱测定小鼠体内甲苯二异氰酸酯(TDI)的代谢产物,并鉴别了其在机体中的代谢途径。TDI色谱条件为Supelco PTETM-5色谱柱(30 mm×0.25 mm×0.25μm),起始柱温90℃保持30 min,以40℃.min-1升至280℃,保持5.25 min;汽化室温度250℃;载气为氦气,流速为1.0μL.min-1。TDI体内代谢产物色谱条件为30+2 mX0.25+0.02 mm 94%甲基、4%乙烯基键合相弹性石英毛细柱,起始温度30℃保持5 min,以8℃.min-1升至280℃,保持5 min。汽化室温度250℃;载气为氦气,流速1.0μL.min-1。质谱条件为电离方式EI,电离能量70 eV,连接管温度280℃,扫描范围35~350μ;进样量1.0μL。结果表明2,4甲苯二异氰酸酯在血液、尿液、粪便中都被代谢成为2,4甲苯二胺。GC-MS法可有效分离并鉴定TDI体内代谢产物。更多还原

【Abstract】 In the present research we used gas chromatography-mass spectrometry(GC-MS)to determine metabolites of toluene diisocyanate(TDI) in mice and deduce the pathway for toluene diisocyanate metabolism in the organism.Conditions for TDI chromatography: Supelco PTETM-5 chromatographic column(30 mm×0.25 mm×0.25 μm);initial column temperature: 90 ℃,which was maintained for 30 min,then the temperature was increased at a rate of 40 ℃·min-1 to 280 ℃,and maintained for 5.25 min;temperature for the vaporizing chamber: 250 ℃;carrier gas: helium flowing at 1.0 μL·min-1.Conditions for chromatography of TDI metabolites in the organism: 94% methyl,4% ethenyl-bonded-phase fused-silica capillary column(30+2 mX0.25 + 0.02 mm);initial column temperature: 30 ℃,which was maintained for 5 min,after and then was increased at a rate of 80 ℃·min-1 to 280 ℃,and maintained for 5 min;temperature for the vaporizing chamber: 250 ℃;carrier gas: helium flowing at 1.0 μL·min-1.Conditions for mass spectrometry: EI for ionization;70 eV for ionization energy;280 ℃ for connecting tube temperature;35-350 μ for range of scanning;and 1.0 μL for sample size.The results showed that 2,4-toluene diisocyanate was metabolized into 2,4-diaminotoluene.Under the conditions selected for GC-MS,TDI metabolites in the organism can be isolated and identified.更多还原