【摘要】 以转基因苹果组培苗嘎拉、王林、富士和乔纳金为试材,研究了影响转基因苹果试管微嫁接苗成活的几个因素。结果表明,以继代培养30d左右、生长健壮的组培苗嫁接成活率较高。适当提高嫁接苗所在培养基中BA质量浓度有利于嫁接成活率的提高,其中以MS+BA1.0mg/L+NAA0.05mg/L培养基中嫁接成活率最高。接穗以带2～4片叶片为宜。同时研究发现,不同转基因品种对嫁接成活率影响不显著。更多还原

【Abstract】 The factors affecting micro-graft in vitro were studied in transgenic lines of Malus domestica cv. Gala, Orin, Fuji, and Jonagold. The results indicated that the higher survival rate was obtained when the plantlets sub-cultured for 30 days and with vigorous adventitious shoots were used. The moderately increasing of the concentration of BA in the medium could increase the survival rate. The optimal medium was MS+BA 1.0 mg/L + NAA 0.05 mg/L for micro-graft in vitro. The scions with 2 to 4 leaves attached were benefit to the grafting. Meanwhile, no difference was found in the survival rate among the apple cultivars used.更多还原