【摘要】 目的:研究高糖诱导牛视网膜血管周细胞凋亡中线粒体细胞色素C(Cyt-C)的变化。方法:以在体外培养3代近融合的牛视网膜毛细血管周细胞为对象,分别在对照组(5.5mmol/L)与高糖各组(15,25,35mmol/L)中孵育6d,透射电镜观察周细胞超微结构改变,TUNEL法检测培养后周细胞的凋亡情况,分光光度计检测周细胞胞质Cyt-C变化。结果:高糖各组周细胞出现明显凋亡改变,凋亡率明显高于对照组(t1=57.450,t2=83.754,t3=136.403,P<0.01);高糖各组胞质Cyt-C浓度明显高于对照组(t1=17.361,t2=17.866,t3=24.072,P<0.01),且Cyt-C浓度与周细胞凋亡率明显正相关(r=0.964,P<0.01)。结论:高糖呈剂量依赖性诱导牛视网膜血管周细胞凋亡,线粒体Cyt-C释放可能参与周细胞凋亡过程。更多还原

【Abstract】 · AIM:To study the changes of mitochondrial cytochrome c in the apoptosis of bovine retinal capillary pericytes(BRPs)induced by high glucose,and explore the potential role of cytochrome c in pericytes apoptosis.· METHODS:BRPs were cultured for 6 days respectively in control group(5.5mmol/L)and in various high glucose groups(15,25,35mmol/L).The cell ultrastructure was observed under electron microscope;the apoptosis of BRPs was studied by in situ TUNEL method;the changes of cytochrome C was assayed by spectrophotometer.· RESULTS:BRPs showed typical changes of apoptosis in the high glucose groups.The apoptotic rate and the concentration of cytoplasmic cytochrome c in high glucose groups were higher than those in the control group.The concentration of cytoplasmic cytochrome c was positively correlated with the apoptotic rate of BRPs(r =0.964,P <0.01).· CONCLUSION:High glucose was dose-dependent and could induce the apoptosis of bovine retinal capillary pericytes.Cytochrome C may participated in the apoptosis.·更多还原