【摘要】 目的研究重症SARS患者肺组织内细胞因子TNF,IL-1α,IL-1β,IL-6,IL-8和IL-10的表达及定位,探讨重症SARS患者弥漫性肺泡损伤的发病机制。方法应用免疫组织化学染色(SABC法)和定量分析方法对8例SARS死亡病例肺组织中TNF,IL-1α,IL-1β,IL-6,IL-8和IL-10进行检测。结果TNF,IL-1α,IL-1β,IL-6,IL-8和IL-10在8例SARS死亡病例肺组织中均有阳性表达,多种细胞均可表达这些细胞因子;与普通肺炎组比较,SARS死亡病例肺组织中细胞因子水平显著升高;SARS死亡病例细胞因子水平在病程不同阶段间的表达有差异。结论TNF,IL-1α,IL-1β,IL-6,IL-8和IL-10可能参与重症SARS患者弥漫性肺泡损伤的发生与发展。更多还原

【Abstract】 Objective To study the expression and location of tumor necrosis factor(TNF),Interleukin1α(IL-1α),IL-1β,IL-6,IL-8 and IL-10 in lungs of severe SARS patients,and to understand the pathogenesis of diffusive alveolar damage in SARS.Methods TNF,IL-1α,IL-1β,IL-6,IL-8 and IL-10 were detected by immunohistochemical SABC method and image analysis in the lung tissues from 8 severe SARS cases.Results TNF,IL-1α,IL-1β,IL-6,IL-8 and IL-10 expressions were detected by immunohistochemical assay in lungs of all severe SARS patients.The results showed that positive staining of all these cytokines were observed in various cell types.The concentration of all the 6 cytokines in lung tissue sections from SARS patients increased significantly,compared with that of the control group,and varied with the different stages of the illness.Conclusion The expression of TNF,IL-1α,IL-1β,IL-6,IL-8 and IL-10 may play an important role in the pathogenesis of diffusive alveolar damage in severe SARS patients.更多还原