【摘要】 目的观察维A酸(RA)对大鼠胃癌前病变增殖细胞核抗原(PCNA,用增殖指数PI表示)和端粒酶活性(用hTERT表示)的影响。方法采用甲硝基亚硝基胍(MNNG)、0.03%雷尼替丁、56℃15%盐水、0.85%脱氧胆酸钠、40%乙醇、饥饱失常等多种因素制作大鼠胃癌前病变模型,免疫组化方法检测正常组、自然恢复组、RA20mg/kg和RA40mg/kg组大鼠胃黏膜组织中PI和hTERT蛋白表达情况。结果正常组和RA40mg/kg组PI、hTERT蛋白阳性率均较自然恢复组低,差异有显著性(P<0.01或P<0.05)。结论维A酸可能是通过抑制端粒酶及细胞增殖活性,发挥逆转胃癌前病变的作用。更多还原

【Abstract】 Objective To observe the effect of retinoic acid(RA) on proliferating cell nuclear antigen (PCNA, represented with proliferating index, PI) and telomerase activity (represented with human telomerase reverse transcriptase,hTERT) in rats with gastric precancerous lesion. Methods The model was established by MNNG together with other factors including 15% 56 ℃ brine,sodium deoxycholate, 40% alcohol stimulus, 0.03% ranitidine, undue hunger,and overeating. PCNA(converted to PI) and hTERT was detected by immunohistochemical method, in normal group, spontaneous recovery group,and RA(20 mg/kg and 40 mg/kg) group.Results PI and hTERT of normal group and RA 40mg/kg groups were lower detected than spontaneous recovery group and was significantly different (P<0.01 or P<0.05). Conclusion RA makes GPL reversed in rates by inhibiting activity of telomerase and cell proliferating.更多还原