【摘要】 采用水相法合成的CdTe半导体纳米粒子作为能量给体,通过Sch iff碱反应将单链DNA连接到表面.采用柠檬酸钠还原氯金酸法制取的Au纳米粒子作为能量受体,通过Au—S键将单链DNA连接到表面.通过DNA链间的杂交,构建了荧光共振能量转移体系(FRET).测定了CdTe-DNA、探针体系和探针体系+目标DNA的荧光强度.结果表明,探针体系的荧光强度最弱,加入目标DNA后,体系荧光增强,表明该体系的构建是成功的.更多还原

【Abstract】 CdTe semiconductor nanoparticles which were synthesized in water phase were taken as the energy donors.CdTe nanoparticles were linked to 5′-NH2-DNA by Schiff′s reaction.Au nanoparticles which were prepared by sodium citrate reduction of chloroauric acids were taken as the energy acceptors.Au nanoparticles were linked to 3′-SH～DNA by Au～S bond.The fluoresscence resonance energy transfer(FRET) probes are generated through hybridization of complementary DNA bound to the QDs and Au nanoparticles.The fluorescence spectra of CdTe-DNA,CdTe-DNA-Au and CdTe-DNA-Au+target DNA were measured.The data show that the fluorescence intensity of CdTe-DNA-Au conjugates was the weakest.When adding a complementary ssDNA to CdTe-DNA-Au conjugates, the fluorescence intensity of the system increased.This result shows that the configuration of FRET was successful.更多还原