【摘要】 目的探讨孕妇外周血中的游离胎儿DNA检测的可行性及其水平变化在早产预测中的价值。方法普通PCR检测17例先兆早产孕妇和20例正常孕妇血浆中Y染色体性别决定基因(SRY),继应用荧光定量PCR技术对有SRY基因表达者的胎儿DNA水平进行定量分析并进一步比较早产孕妇组和正常孕妇组间DNA水平的差异。结果17例先兆早产患者及20例对照组中各有9例、10例出现SRY阳性信号。先兆早产组的胎儿DNA水平明显高于正常对照组(P<0.01)。结论孕妇外周血中的游离胎儿DNA可望作为预测早产的指标之一。更多还原

【Abstract】 Objective To evaluate the possibility of detecting the cell-free fetal DNA in maternal plasma and its value as a design of the preterm labor prediction.Methods The normal PCR was used to analyze the cell-free SRY fetal DNA in the plasma of 17 preterm labor women and 20 normal pregnant women.Then,the fluorescent quantitative PCR was used to comparing the fetal DNA level in the plasma of preterm labor women with that of normal pregnant women.Results Among the preterm labor and normal pregnant women,there are 9 and 10 women had male fetus,respectively.The fetal DNA level in plasma of the preterm labor women was much higher than that of normal women(P<0.01).Conclusion The cell-free fetal DNA in maternal plasma may be a design for the prediction of preterm labor.更多还原