【摘要】 目的研究吸附性载体上微量血痕的DNA提取及其检验。方法用Chelex-100法、QIAamp MiniKit、及QIAamp Mini Kit改良法提取吸附性载体上微量血痕中的DNA,进行PCR扩增及STR检验。结果采用Chelex-100法及QIAamp Mini Kit的分型成功率很低;采用QIAamp Mini Kit的改良法能较好的得到分型图谱。结论采用QIAamp Mini Kit的改良法能较好的提取吸附性载体上微量血痕的模板DNA。更多还原

【Abstract】 Objective To study the extraction and analysis of DNA from trace bloodstains on the adsorbent object.Methods DNA was extracted by Chelex-100,QIAamp Mini Kit,and modified QIAamp Mini Kit,respectively.The extracted DNA was amplified and short tandem repeat(STR) loci were genotyped by multiplex PCR procedures,and then analyzed by ABI 3100 Genetic Analyzer.Results The STR loci could be better genotyped with modified QIAamp Mini Kit,compared to poorer genotyping results obtained with Chelex-100 extraction method and QIAamp Mini Kit.Conclusion Our data indicate that DNA extracted from trace bloodstains on adsorbent object with modified QIAamp Mini Kit serves a better DNA template for amplification and genotyping.更多还原