【摘要】 目的探讨大鼠脑挫伤后低氧诱导因子-1α(HIF-1α)和胰岛素样生长因子-1(IGF-1)表达的变化规律。方法通过组织学和免疫组织化学法(SABC法)对正常及伤后不同时间HIF-1α、IGF-1蛋白的表达进行检测,并作统计学分析。结果正常对照组神经细胞偶见HIF-1α表达,IGF-1表达呈弱阳性。脑挫伤后HIF-1α和IGF-1的表达主要位于大脑皮质、脑干和海马的神经元细胞和神经胶质细胞中,并以神经胶质细胞为主。伤后2h挫伤区周围神经细胞可见HIF-1α蛋白阳性表达增加,24h达高峰,14d后恢复正常;伤后6hIGF-1蛋白阳性表达增加,3d达高峰,14d后恢复正常。各实验组与对照组比较有显著性差异(P<0.05)。结论脑损伤后HIF-1α、IGF-1蛋白的表达具有一定的时间规律性,可望用于法医病理学推断脑损伤时间。更多还原

【Abstract】 Objective To investigate the expressions of hypoxia induced factor-1α(HIF-1α) and insulin-like growth factor-1 (IGF-1) at different post brain injury intervals after cerebral contusion. Methods An experimental rat model of severe cerebral contusion was used and the expressions of HIF-1α and IGF-1 proteins at different post brain injury intervals were studied with immunohistochemistry and imaging analysis technique. Results The expressions of both the HIF-1α and IGF-1 proteins were detected in neurons and glial cells in cerebral cortex, brain stem, and hippocampus. The expression level of HIF-1α started to increase in the neural cells around the injured areas at 2 hours, reached its maximal level at 24 hours, and returned to its baseline level at 14 days after the injury. The expression level of IGF-1 protein started to increase at 6 hours, reached its maximal level at 3 days, and returned to its baseline level at 14 days after the injury. Conclusion The level changes of post injury HIF-1α and IGF-1 expressions in rats may be used for estimation of post brain contusion interval.更多还原