【摘要】 目的研究大鼠死亡后腓肠肌内糖原含量变化与死亡时间之间的关系。方法采用断颈、机械性窒息、失血性休克三种方法分别处死大鼠,于死亡后0﹑0.5﹑1﹑1.5﹑2﹑3﹑4﹑8﹑12﹑18和24h时间点取腓肠肌,利用PAS染色方法并结合计算机图像分析技术分析糖原含量变化。结果大鼠腓肠肌内糖原含量和阳性面积随死亡后时间的延长而呈线性减少。结论腓肠肌内糖原含量和阳性面积可作为死亡时间推断的参考指标。更多还原

【Abstract】 Objective To study the relationship between the content of glycogen in gastrocnemius of rat and postmortem interval (PMI). Methods The gastronomies muscles were collected at 0,0.5,1,1.5,2,3,4,6,8,12,18 and 24 hours from rats sacrificed by bleeding, suffocating, and neck breaking, respectively. The changes of glycogen content in these muscles were analyzed by PAS staining and imaging technique. Results The content of glycogen in the rat’s gastrocnermius muscle showed a continuous linear decrease in correlation with prolonged postmortem intervals. Conclusion Our data suggest that the changes of glycogen content in rat’s gastrocnemius can be used as one of the references for estimation of the postmortem interval.更多还原