【摘要】 观察了超大剂量6MeV电子线照射后大鼠脑组织NSE、S-100蛋白的动态变化。对成熟的(Sprague-dawle,SD)大鼠用6MeV电子线进行10Gy、20Gy和30Gy全脑单次照射,应用免疫组织化学法测定大鼠脑损伤后不同时间、不同剂量脑组织中稀醇化酶(Neuro-specific enolase,NSE)、S-100蛋白的相对含量。60只大鼠随机分为对照组和实验组,用6MeV电子线对实验组大鼠进行20Gy全脑单次垂直照射,分别于照射后1d、7d、14d和30d处死大鼠,取其脑组织,用免疫组织化学法测定NSE、S-100蛋白的相对含量,并与对照组进行比较。在上述时间点,脑内海马区S-100和NSE表达有时间规律,照射后7dS-100蛋白的表达和照射后24hNSE的表达组间存在一定差别。与对照组相比,实验组大鼠脑组织中NSE表达显著下降(p<0.05),S-100表达显著升高(p<0.05)。在照射后7d,上述各指标变化的幅度为30Gy组>20Gy组>10Gy组。电离辐射可诱导脑组织中S-100蛋白的表达,同时下调NSE在神经元中的表达,脑组织中S-100和NSE表达水平的变化可作为辐射诱导的急性脑损伤的敏感指标。更多还原

【Abstract】 The work is to study the expression of NSE and S-100 protein in SD rat brain tissue irradiated by 6MeV electron beam. The whole brain of the Sprague-Dawley rats were irradiated with single dose of 10Gy, 20Gy and 30Gy by 6MeV electron. Immunohistochemical method was used to measure the contents of S-100 and NSE in brain tissue. One-ANOVA was used for analysis. The expression of S-100 and NSE after whole brain irradiation was time-dependent. Compared with that of control group, there was an increase of expression of S-100 D7 after irradia-tion during the observed time (p<0.05). On the contrary, there was a decrease of NSE 24h after radiation, disappear-ing nearly at D30 (p<0.05). The indexes above were changed with the different dose levels at 7d after radiation. Ra-diation can induce the expression of S-100, as well as inhibit the expression of NSE on radiation-induced acute brain injury. The changes of the expression of S-100 and NSE on the brain can act as indexes of acute brain injury induced by radiation.更多还原