【摘要】 为了研究低浓度空气离子对微生物的效应,利用低浓度空气离子对固体培养基上Streptomyces aver-mitilis作用,分析了其次生代谢产物产生时间早晚,各组分含量变化,并且初步研究有效成分含量提高的机理。用浓度为5×103/cm3的空气负离子连续十天处理固体培养基上的阿维链霉菌,经过高效液相色谱(High performance liquid chromatography,HPLC)检测后发现:经过处理,该菌株提早四天到达阿维菌素(Avermectins,AVM)产素平台期,有效成分B1a和B1b提高约20%,而无效成分含量无显著提高。经过分析,低浓度空气负离子可有效缩短该菌种的发酵周期,提高有效成分含量,刺激次生代谢过程。更多还原

【Abstract】 Effect of low concentration negative air ions on Streptomyces avermitilis on solid culture medium was studied. The microorganism was treated consecutively with negative air ions of 5×103/cm3 in 10 days. It was found that under the treatment, Streptomyces avermitilis reached the plat-stage production 4 days earlier than the con- trol. Moreover, quantities of both effective contents of B1a and B1b increased by about 20%, while no remarkable changes in the ineffective contents were observed. Repeated experiments prove that the negative ion treatment is a useful way to shorten long fermentation cycle and to increase antibiotic production.更多还原