【摘要】 目的:建立高效液相色谱法测定左炔诺孕酮油水分配系数的方法。方法:Symmetry(?)色谱柱(150 mm×3．9 mm,5．0μm),乙腈与水为流动相(体积比为45:55),流速1．0 mL／min,检测波长240 nm。结果:在0．0216～1．0820μg／mL的范围内呈良好的线性关系,相关系数r=0．9999,方法平均回收率98．75％～100．07％;日内和日间RSD分别小于1．25％和1．60％,检出限为1．0 ng／mL。结论:方法简便,快捷,适应于左炔诺孕酮油水分配系数的测定。更多还原

【Abstract】 Objective: To determine oil-water partition coefficient of levonorgestrel by HPLC. Methods: The Symmetry(?) was used as separation column (150mm×3. 9mm, 5. 0μm) with acetonitrile-water(45 : 55) as mobile phase (1.0mL/min) and detection at 240nm. Results: The linear range is 0. 0216～1. 082μg/mL ( r = 0. 9999) with detection limit of 1. 0ng/mL. The recovery is 98.75%～100.07%. The intraday and interday precisions are <1. 25% and <1. 60%, respectively. Conclusion: The method is rapid, simple and accurate and is suitable for determining oil-water partition coefficient of levonorgestrel.更多还原