【摘要】 建立了一种用于分析太子参中环肽Pseudostellarin B的高效液相色谱法。该方法采用反相C18柱:流动相A为水,流动相B为乙腈,梯度洗脱,流速为1.0 mL/min,紫外检测波长为203 nm,柱温为30℃,外标法定量。结果表明,在0.5~20μg范围内标准曲线有良好的线性关系(r=0.9995),加标回收率为96.7%~104.5%,方法的精密度良好(平均RSD<0.5%)。该方法可用于实际样品的测定。更多还原

【Abstract】 A high performance liquid chromatographic（HPLC） method for the determination of Pseudostellarin B（cyclic peptide） in Taizishen（Pseudostellaria heterophylla（Miq.） Pax） has been established.The separation was carried out on a C₁₈ analytical column at 30 ℃ with a linear gradient elution of water（A）-acetonitrile（B）.The flow-rate of 1.0 mL/min and UV detection at 203 nm were used in this study.The experimental results indicate that a good linear range of 0.5～20 μg（r=0.9995） could be obtained.The recovery test for Pseudostellarin B was in the range of 96.7%～104.5%.The relative standard deviation was less than 1.8%.The results indicate that the method is simple,rapid and accurate for the determination of Pseudostellarin B in Taizishen.The method is recommended to apply to the real sample analysis.更多还原