【摘要】 在碱性介质中,6-巯基嘌呤(6-MP)与过氧化氢反应可在405 nm波长处产生微弱的荧光,微量铜(Ⅱ)的存在能大大增敏其荧光强度。据此,建立了一种高灵敏测定6-MP的荧光光度分析新方法。在优化的条件下,体系的荧光强度与6-MP的浓度在2.1×10-8~1.7×10-5mol.L-1范围内呈线性关系,检出限为7.1×10-9mol.L-1。方法用于片剂中6-MP含量的测定,其结果与药典方法对照,令人满意。更多还原

【Abstract】 In basic media,6-mercaptopurine reacts with hydrogen peroxide to form a new fluorescent compound,which possesses a weak emission wavelength of 405 nm.Its fluorescence intensity can be enhanced remarkably when trace Cu2+ exists as catalyst.Based on this,a novel spectrofluorimetric method with high sensitivity was developed for the determination of 6-mercaptopurine.Under the optimal conditions,the calibration graphs are linear over the range of 2.1×10-8~ 1.7×10-5 mol·L-1 with a detection limit of 7.1×10-9 mol·L-1.The proposed method was successfully applied to determine 6-mercaptopurine in pharmaceutical preparations,and the results obtained were in good agreement with the results obtained by pharmacopoeia method.更多还原