【摘要】 采用毛细管区带电泳紫外检测法(CZE-UV)同时测定中药莲须中槲皮素、木犀草素、山萘酚、异槲皮甙4种有效成分。研究了缓冲溶液的离子浓度、pH值和电压对分离度和迁移时间的影响,得到了最佳分离实验条件。在离子浓度为40mmol/LNa2B4O7缓冲溶液(pH9.0)中,分离电压为16kV,波长为254nm时,槲皮素、木犀草素、山萘酚、异槲皮甙4种物质在10min内得到了良好的分离测定,其检出限分别为5.0、6.7、4.5和6.0mg/L。本方法应用于实际样品的测定,结果令人满意。更多还原

【Abstract】 The determination of quercetin,luteolin,kaempferol and isoquercitrin in the Chinese herbal-stamen nelumbinis by capillary zone electrophoresis(CZE) with ultraviolet detection(UV) is reported.The analytical conditions were optimized through investigation of the effects of buffer pH,buffer concentration and applied voltage.The optimized conditions are as following:40 mmol/L Na2B4O7 solution(pH=9.0),working voltage of 16 kV and detection wavelength of 254 nm,the detection limits were 5.0,6.7,4.5 and 6.0 mg/L,respectively.These four analytes can be well-separated in 10 min.更多还原