【摘要】 利用定量聚合酶链式反应来检测PCR起始阶段的古DNA模板数是鉴定古DNA保存状况以及确保其真实性的有效方法之一。采用SYBRgreenI法对距今3800年左右的新疆小河墓地出土的5例样本线粒体DNA的3个不同长度片段(138bp、209bp和363bp)及核DNA(AMG基因)进行定量。线粒体DNA定量结果显示:小河样本古DNA扩增效率与扩增序列长度成负相关性,只有138bp的结果高于每微升150拷贝。虽然大部分小河样本的AMG基因定量结果都在30拷贝以内,仍然可以进行核DNA的STR或SNP分析。本实验首次通过定量PCR的方法,证明在同一个体中的牙齿中的DNA拷贝数要高于骨骼中的。更多还原

【Abstract】 Human DNA quantification has became an essential analysis to ensure the preservation of ancient samples and the authentication of the result in ancient DNA research.Synergy brands(SYBR)green Ⅰ Real-time PCR was used to quantify five samples which were excavated in Xiaohe cemetery by using the SYBR GREEN PCR Master Mix in Gene Amp@ 5700 Sequence Detector.Three different fragment sizes(138 bp,209 bp and 363 bp)within the hypervariable region Ⅰ(HV1)of the mitochondiral deoxyribonuclear acid(mtDNA)and the amelogenin(AMG)gene(121/115 bp)of the nuclear DNA were analyzed.The result showed an inverse correlation between amplicon length and amplification efficiency,only the results of 138 bp were all higher than 150 copies/μL.Although most results of the AMG gene were lower than 30 copies/μL,short tandem repeat(STR)and single nucleotide polymorphism(SNP)of nuclear DNA could be analyzed too.The research also showed that the teeth samples had more DNA copies than bone samples.更多还原