【摘要】 采用毛细管电泳-安培检测法建立一种简单、快速、有效的同时分析抗癌药物2-氨基-6-巯基嘌呤和8-氮杂鸟嘌呤的新方法.在长50 cm、内径为25μm的未涂层毛细管中,采用20 mmol/L磷酸盐(pH为7.0)缓冲液作为运行液,当分离电压为21 kV时,两组分在12 min内达到基线分离.在上述最佳分离条件下,当电极电位为1.050V、进样时间为10 s时,2-氨基-6-巯基嘌呤和8-氮杂鸟嘌呤的峰电流和浓度之间呈良好的线性关系,其相关系数分别为0.999 20、.999 0,检测限分别为3.0×10-7、2.0×10-7mol/L.7次重复实验2-氨基-6-巯基嘌呤和8-氮杂鸟嘌呤的日间峰电流RSD分别为2.44%3、.46%.利用该法检测了牛血清蛋白中的2-氨基-6-巯基嘌呤和8-氮杂鸟嘌呤,回收率分别为100%-113%,93.0-102%.更多还原

【Abstract】 A end-column amperometric detection method with capillary electrophoresis for determination of 2-amino-6-mercaptopurine and 8-azaguanine has been established.A 50 cm length of 25 μm i.d.,360 o.d.μm uncoated fused-silica capillary was used.The detection conditions were optimized and the electrochemical behavior was observed.Under the optimal conditions: detection potential of 1.050 V(vs.Ag/AgCl),operating voltage of 21 kV,sample injection of 10 s at 21 kV and 20 mmol/L phosphate solution at pH 7.0 as buffer,the detection limits were 3.0×10-7 and 2.0×10-7 mol/L,and the calibration curve showed good linearity with r=0.9992 and r=0.9990 for 2-amino-6-mercaptopurine and 8-azaguanine,respectively.The daytime RSD of reproducibility for peak current was 2.44% and 3.46%,respectively.The method was successfully applied to determine serum samples.The recovery was found in the rang of 100%～113% for 2-amino-6-mercaptopurine and 93.0%～102% for 8-azaguanine,respectively.更多还原