【摘要】 目的研究可溶性血管内皮生长因子受体-1(sFlt-1) mRNA在正常妊娠和子痫前期胎盘中的表达差异及相应的母体外周血中sFlt-1水平的变化。方法选取15例子痫前期孕妇(子痫前期组)和14例孕周与之相匹配的血压正常妊娠孕妇(对照组),用ELISA方法测定其外周血中sFlt-1的水平;并应用实时荧光定量PCR测定两组胎盘组织中sFlt-1 mRNA水平。结果外周血中sFlt-1的水平子痫前期组明显高于对照组,分别为(16.9±8.32)μg/L、(5.48±2.09)μg/L,两者差异有显著性(P<0.05)。sFlt-1 mRNA在两组胎盘中均有表达,在子痫前期组的表达明显高于对照组的表达分别为(4.11±4.09)、(1.69±1.61),两者差异有显著性(P<0.05)。子痫前期组血清sFlt-1水平与24h尿蛋白定量呈明显正相关(r=0.741,P<0.05)。结论sFlt-1 mRNA在子痫前期组孕妇的外周血中及胎盘组织表达升高,可能与子痫前期的病因及病理生理有关。更多还原

【Abstract】 Objective To investigate the mRNA expression of sFlt-1 in placenta and serum in patients with preeclampsia. Methods The expression of sFlt-1 mRNA was detected by two-step real-time quantitative polymerase chain reaction (real-time PCR). Fifteen and fourteen placental specimens were collected from preeclampsia group and healthy control with matched gestational age, respectively. Maternal sFlt1 concentration in serum before labor was measured by enzyme linked immunosorbent assay (ELISA). Results Maternal serum sFlt-1 was significantly higher in preeclampsia group than control, (16.9±8.32) μg/L vs (5.48±2.09)μg/L, P<0.05.The sFlt-1 mRNA expression could be detected in both groups. Compared with control group, sFlt-1 mRNA expression was significantly higher in preeclampsia placentas, (1.69±1.61) vs (4.11±4.09), P<0.05. Positive relationship was observed between the level of sFlt-1 and the 24-hour proteinuria in women with preeclampsia (r=0.741, P<0.05). Conclusion High maternal serum sFlt-1 and over expression of sFlt-1 mRNA in preeclampsia placenta may relate to the pathogenesis and pathophysiology of preeclampsia.更多还原