【摘要】 目的建立产超广谱β-内酰胺酶(ESBL)确证的快速微量检测法,评价该方法在筛检工作中的应用效果。方法以灭菌的96孔酶标板建立ESBL微量快速确证试验,用已知59株产ESBL大肠埃希菌、33株非产ESBL大肠埃希菌和质控菌株进行试验的真实性、可靠性和收益评价。结果重复试验符合率100%;用酶标仪自动判读敏感度达到或超过94.92%,特异度和阳性预测值均为100%,与美国国家实验室标准化研究所(CLSI)推荐标准检测方法所得到的结果差别无统计学意义(P>0.05)。结论ESBL微量确证试验具有良好的真实性、可靠性和检测效益;操作简便、经济、快捷。更多还原

【Abstract】 Objective To establish a rapid micromethod for phenotypic confirmatory test of extended-spectrum β-lactamases(ESBL) producing bacteria and to evaluate the efficacy of the method in screening programme. Methods The advanced rapid micromethod for the confirmation of ESBL producing strains was designed using sterile 96-well plastic plates based on the principal of ESBL phenotypic confirmatory test recommended by the current Clinical and Laboratory Standards Institute(CLSI). The test-retest reliability and validity, as well as the effectiveness of the micromethod were assessed by the randomized controlled trial consisting of 59 ESBL positive E.coli, 33 ESBL negative E.coli, and 2 quality control strains. Results Sufficiently high test-retest reliability and validity was achieved with a coincident rate of 100%, a specificity and positive predictive value of 100%, and a sufficient sensitivity beyond 94.92% by the automatic enzyme-labeled assay at an optical density of 570, 600 or 630 nm. Conclusion An extraordinary efficiency for the rapid micromethod of phenotypic confirmatory test of ESBL was established.更多还原