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水稻裂颖突变体srg花器官突变的形态发生及遗传分析

Genetic analysis and morphogenesis of floral mutant split rice glume

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【作者】 梁康迳王俊兰姚小兰林晶陈仁财柯庆明林文雄

【Author】 LIANG Kang-jing,WANG Jun-lan,YAO Xiao-lan,LIN Jing,CHEN Ren-cai,KE Qing-ming,LIN Wen-xiong(College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

【机构】 福建农林大学作物科学学院福建农林大学作物科学学院 福建福州350002福建福州350002

【摘要】 在水稻Lemont×Dular的重组自交系群体(RILs,F10)中发现了裂颖突变体(split rice glume,srg).利用扫描电子显微镜观察野生型水稻和裂颖水稻的颖花原基分化情况,结果发现,突变体水稻的幼穗在形成内、外稃原基后,有些颖花在形成浆片后并不是直接发育形成雄蕊原基,而是在外稃或内稃原基内侧又分化出浆片原基;有些颖花原基从中间缢裂,缢裂的颖花原基又分别形成类稃片的结构,颖花发育后期,6枚雄蕊发育畸形,并且观察到不同数目的雄蕊.同时以突变体作母本,Dular、Lemont为父本配制杂交组合进行遗传分析,F2表型和χ2测验结果表明,该突变性状受单基因隐性控制.

【Abstract】 A floral organ mutant,split rice glume(srg),was investigated in rice recombinant inbred lines population(RILs,F10),which was derived from rice variety Lemont×Dular.The morphogenesis of floral organs in both srg mutant and wild-type rice Dular(Oryza sativa L.) was observed using the scanning electron microscope(SEM).The results showed that the floral primordia were differentiated under irregular pattern in srg mutant.After regular formation of lemma and palea primordia,the floral primordium of srg formed lodicule primordia inner to both lemma and palea,followed by the formation of stamens and carpel.Alternatively,floral primordia in srg were split to form two or more floral primordia.The number and position of stamens and carpels primordia were mal-formed.Two F2 populations were made by crossing srg mutant with two rice varieties Lemont and Dular,respectively,and genetic analyses showed that the mutant trait was controlled by a single recessive gene.

【关键词】 水稻srg花器官扫描电子显微镜遗传
【Key words】 ricesrgfloral organscanning electron microscopegenetics
【基金】 福建省自然科学基金资助项目(B0510015)
  • 【文献出处】 福建农林大学学报(自然科学版) ,Journal of Fujian Agriculture and Forestry University(Natural Science Edition) , 编辑部邮箱 ,2007年03期
  • 【分类号】S511;Q943
  • 【被引频次】5
  • 【下载频次】214
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