【摘要】 克隆番茄ACC氧化酶1(LeACO1)基因特异片段,以此作为探针,与经过干旱和干旱后复水、淹水、伤害、不同温度等胁迫环境及乙烯处理的番茄总RNA进行Northern杂交,杂交结果表明,LeACO1基因的表达被干旱抑制,被复水、外源乙烯强烈诱导;淹水处理对叶片中该基因的表达无影响,而强烈诱导其在茎中的表达;37℃高温处理可以诱导LeACO1基因在番茄茎中的表达;伤害对该基因的表达没有明显影响.更多还原

【Abstract】 The specific ACC oxidase 1 gene fragment of tomato was cloned and prepared for a probe which was used to hybridize with total RNAs that were extracted from the materials treated with ethylene and different environmental stresses,such as drought,water recovery,flood,wound and temperature,respectively.Northern blot analysis indicated that the expression of LeACO1 was restrained by drought,while strongly induced by water recovery and exogenous ethylene.After treated by flood stress,the expression level of LeACO1 in leaves had no distinctively changes,while its expression level in stems was rapidly increased.Moreover,the expression of LeACO1 in tomato stems could be induced at the temperature of 37 degree.In addition,wounding had no influence on the expression of LeACO1.更多还原