【摘要】 分别研究了封闭液、血清稀释液及酶标二抗稀释液中BSA成分对牛结核抗体ELISA检测的影响。结果显示,酶标二抗稀释液中BSA的存在使ELISA检测值普遍偏低,且BSA的含量越高,检测值越低;为了提高ELISA检测值,使其达到理想检测效果,必须提高酶标二抗的使用浓度。BSA中可能存在的牛血清抗体杂质对酶标二抗起到了“中和”作用,降低了酶标二抗与目标抗体结合的实际浓度,使检测结果普遍偏低。更多还原

【Abstract】 In this study,bovine serum albumin(BSA) was used as components of blocking buffer,diluents for serum and enzyme-labeled secondary antibody,respectively.And the effects of BSA on ELISA which was used to detect antibodies of Mycobacterium bovis was discussed.The component of BSA in diluent for enzyme-labeled secondary antibody was found to significantly lower the read of OD630 in ELISA test,and with the increasing of BSA in diluent for enzyme-labeled secondary antibody,the read of OD630 in ELISA test fell.In order to heighten the read of ELISA test and reach an ideal detection effects,we had to heighten concentration enzyme-labeled secondary antibody.Based on the above research,we got that there was some antibody of bovine serum in BSA.The enzyme-labeled secondary antibody was counteracted by these antibody,and the concentration of which was reduced when it reacted with target antibody.Then read of ELISA test was lowered.更多还原