【摘要】 根据GenBank已登录的猪囊尾蚴膜联蛋白B2基因序列,设计合成1对特异性引物,应用反转录-聚合酶链反应(RT-PCR)技术从猪囊尾蚴中扩增出膜联蛋白B2基因,将其克隆至pcDNA3.1表达载体上,经酶切鉴定和基因测序表明,目的基因AnnexinB2已正确地整合至表达质粒中,成功构建了膜联蛋白B2基因的真核表达载体。更多还原

【Abstract】 According to the published sequence of annexin B2 gene of Cysticercus cellulosae,a pair of primers were designed and synthesized.The annexin B2 gene was amplified by RT-PCR method from Cysticercus cellulosae,and cloned into pcDNA3.1 vector.The annexin B2 gene was sequenced and compared with the published sequence of annexin B2 gene of Cysticercus cellulosae in the GenBank.The annexin B2 gene was successfully conformed into the expression plasmid.The eukaryotic expression plasmid containing annexin B2 gene was successfully constructed and was helpful for the studies of function of annexin B2.更多还原