【摘要】 从感染鸡马立克病病毒血清Ⅰ型(MDVⅠ)814株的鸡胚成纤维细胞(CEF)中提取病毒总DNA,以其为模板,根据GenBank中MDVⅠGA株基因组gE、gI、gp82基因序列,设计并合成3对特异性引物,用PCR方法分别扩增了814株的gE、gI、gp82基因,并将扩增的基因片段克隆到pMD18-T载体中,进行序列测定,应用DNA Star软件分析814株gE、gI、gp82基因核苷酸序列,并与已发表的MDVⅠ毒株序列进行了比较。结果表明,不同MDVⅠ毒株的gE、gI、gp82基因同源性很高,814株与已发表的MDVⅠ毒株的gE、gI、gp82基因核苷酸序列同源性分别在99.4%、98.9%和99.6%以上。更多还原

【Abstract】 Three sets of PCR primers were designed according to the gE,gI,gp82 genes of genome sequence of strain GA of Marek’s disease virus serotype-1(MDVⅠ).The three genes of strain 814 of MDVⅠ were amplified from CEF infected with strain 814 of MDVⅠ,and the PCR products were cloned into pMD18T vector,respectively.The genes were sequenced.Their nucleotide sequences were analyzed by DNAStar software.Results indicated that homologies of genes gE,gI,gp82 are more than 99.4%,98.9%,99.6%,respectively between strain 814 and other strains of MDVⅠ.更多还原